Assessment of Antioxidant Activities of Flaxseed (Linum Usitatisimum L.) and Fennel Seed (Foeniculum Vulgare Mill.) Extracts

Flaxseed and fennel seeds are rich sources of different types of phenolics such as lignans,
phenolic acids, flavonoids, phenylpropanoids, and tannins. The present study was carried
out to develop and optimize a technique for isolation and quantification of
secoisolariciresinol diglucoside (SDG) from flaxseed and Anethole from fennel seeds and
to assess the antioxidant activities of flaxseed (Linum usitatisimum L.) and fennel seeds
(Foeniculum vulgare Mill.) extracts. The percentage of extract in fennel seeds and
flaxseed was 5.72 and 6.31 %, respectively. Using HPLC, the predominant constituent in
fennel seed was determined to be anethole (11.30±0.25 mg/g) and in flaxseed, it was
secoisolariciresinol diglucoside (SDG) (8.35±0.52 mg/g) compared to the respective
standards solutions. Using SPSS, the obtained data from each parameter were statistically
analyzed. There are numerous biological and pharmacological effects of trans-anethole. For
antioxidant activity, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) showed higher results with acetone(80%), with fennel seed and flaxseed is 7.28±0.17 and 8.29±0.23 mM TE/g. while FTC
method it was maximum for distilled water extract of fennel seed (48.35±0.19%) and
Flaxseed (65.09±0.72%) and by β-carotene bleaching method, water extract of fennel seed
and flaxseed also showed maximum antioxidant activity i.e. 83.06±0.02% and
86.23±0.38%. Additionally, it is used as a masking agent in food products as a flavoring agent.
According to the findings of this study, fennel seeds and flaxseed contain bioactive compounds
that may assist in the treatment of a number of chronic diseases.