Jour of Nutr. Biochem., 2012, Volume 24; Issue 6: Pages 1041-1052.

Effects of ALA, EPA and DHA in high-carbohydrate, high-fat diet-induced metabolic syndrome in rats.

Poudyal, H. Panchal, SK. Ward, LC. Brown, L.

Key Findings:

ALA attenuated most structural and functional changes caused by a high fat diet and caused lipid redistribution from the abdominal cavity. ALA, EPA and DHA produced similar tissue lipid profiles. Both flax seed and fish oil offset the effects of trans-fatty acids and this study shows a decreased trans-fatty acids in tissues following treatment with n-3 fatty acids.  ALA, EPA and DHA all reduced cardiac fibrosis, hepatic steatosis and inflammation in both the heart and the liver.

ABSTRACT:

We compared the cardiovascular, hepatic and metabolic responses to individual dietary n-3 fatty acids (α-linolenic acid, ALA; eicosapentaenoic acid, EPA; and docosahexaenoic acid, DHA) in a high-carbohydrate, high-fat diet-induced model of metabolic syndrome in rats. Additionally, we measured fatty acid composition of plasma, adipose tissue, liver, heart and skeletal muscle in these rats. The same dosages of ALA and EPA/DHA produced different physiological responses to decrease the risk factors for metabolic syndrome. ALA did not reduce total body fat but induced lipid redistribution away from the abdominal area and favorably improved glucose tolerance, insulin sensitivity, dyslipidemia, hypertension and left ventricular dimensions, contractility, volumes and stiffness. EPA and DHA increased sympathetic activation, reduced the abdominal adiposity and total body fat and attenuated insulin sensitivity, dyslipidemia, hypertension and left ventricular stiffness but not glucose tolerance. However, ALA, EPA and DHA all reduced inflammation in both the heart and the liver, cardiac fibrosis and hepatic steatosis. These effects were associated with complete suppression of stearoyl-CoA desaturase 1 activity. Since the physiological responses to EPA and DHA were similar, it is likely that the effects are mediated by DHA with EPA serving as a precursor. Also, ALA supplementation increased DHA concentrations but induced different physiological responses to EPA and DHA. This result strongly suggests that ALA has independent effects in metabolic syndrome, not relying on its metabolism to DHA. (Authors abstract)

 

 

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