Key Findings
Bioactive peptides are small segments of amino acids with physiological properties. They can be obtained and isolated after enzymatic hydrolysis. Peptide fractions recovered from flaxseed protein after hydrolysis show both anti diabetic activity and ACE inhibitory activity (antihypertensive activity). This paper provides an overview of the technologies that can be used to produce the bioactive peptides. Such isolates from flaxseed have great potential for many disease reducing applications – especially in the area of blood pressure lowering.
ABSTRACT
Flaxseed protein hydrolysate has been fractionated by electrodialysis with two ultrafiltration membranes (20 and 50 kDa) stacked in the system for the recovery of two specific cationic peptide fractions (KCl-F1 and KCl-F2). After 360 min of treatment, peptide migration increased as a function of time in KCl compartments. Moreover, the use of two different ultrafiltration membrane allowed concentration of the 300 to 400 and 400 to 500 Da molecular weight range peptides in the KCl to F1 and KCl to F2 fractions, respectively, compared to the initial hydrolysate. After mass spectrometry analysis, higher amounts of low molecular weight peptides were recovered in the KCl to F2 compartment while relatively higher molecular weight peptides were more detected in the KCl to F1 compartment. Amino acid analysis showed that His, Lys and Arg were especially concentrated in the KCl compartments. Finally, glucose transport assay demonstrated that the KCl to F2 fraction increased glucose uptake while oral administration of KCl to F1 and final FPH decreased systolic blood pressure. (Authors abstract)
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